9/12/2023 0 Comments Klenow fragment ligation![]() Preparation of the InsertĪn Insert is any desired fragment of DNA that you want to clone into a vector/plasmid. Selection of transformants and other downstream processes.Removal of the terminal phosphate of the linearized vector (phosphatase reaction).This also increases screening efforts to get the clone with the desired orientation.īlunt-end cloning involves the following steps. It is challenging as the probability of generating a clone with multiple inserts with the right orientation is low.In certain instances where the insert concentration is high, a ligated vector can have more than one insert.Additional steps are needed to avoid self-ligation (discussed below). Blunt-end cloning is prone to vector self-ligation.This results in a little chance for a stable association between the insert and the vector, resulting in a lower recombination efficiency (10-100x) than sticky-end cloning. In blunt-end cloning, both inserts and vectors do not have complementary 3’ or 5’ overhangs.Non-directional cloning generates only 50% of inserts with the proper orientation.It is used for sub-cloning, sequencing, building libraries of DNA molecules, and expressing coding and non-coding RNA.It does not require a complementary sequence between the insert and the This property makes it a truly universal cloning method. Blunt-end cloning has an advantage over other cloning methods (TA-cloning or traditional restriction enzyme-based sticky-end cloning).Blunt-end cloning involves simple design of the primers (without any extra bases at the 5’ end of the primer).The T4 DNA ligase utilizes ATP to make a phosphodiester bond between the 3’ hydroxyl group of one DNA strand and the 5’ phosphate group of another DNA strand.Ī detailed mechanism of ligation is discussed here. During the transient association of the ends of the vector and the insert, the DNA ligase enzyme seals the joints. The exo-Klenow fragment is used in some fluorescent labeling reactions for microarray, and also in dA and dT tailing, an important step in the process of ligating DNA adapters to DNA fragments, frequently used in preparing DNA libraries for Next-Gen sequencing.In blunt-end cloning, both the vector and the insert contain blunt-ends. This form of the enzyme is called the exo- Klenow fragment. This results in forms of the enzyme being expressed that retain 5' → 3' polymerase activity, but lack any exonuclease activity (5' → 3' or 3' → 5'). This problem can be overcome by introducing mutations in the gene that encodes Klenow. Just as the 5' → 3' exonuclease activity of DNA polymerase I from E.coli can be undesirable, the 3' → 5' exonuclease activity of Klenow fragment can also be undesirable for certain applications. The Klenow fragment was also the original enzyme used for greatly amplifying segments of DNA in the polymerase chain reaction (PCR) process, before being replaced by thermostable enzymes such as Taq polymerase. Filling in receded 3' ends of DNA fragments to make 5' overhang blunt.Synthesis of double-stranded DNA from single-stranded templates.The Klenow fragment is extremely useful for research-based tasks such as: coli makes it unsuitable for many applications, the Klenow fragment, which lacks this activity, can be very useful in research. 5' → 3' polymerase activity, and 3' → 5' exonuclease activity).īecause the 5' → 3' exonuclease activity of DNA polymerase I from E. coli is cleaved by subtilisin retains the 5' → 3' exonuclease activity but does not have the other two activities exhibited by the Klenow fragment (i.e. The other smaller fragment formed when DNA polymerase I from E. First reported in 1970, it retains the 5' → 3' polymerase activity and the 3’ → 5’ exonuclease activity for removal of precoding nucleotides and proofreading, but loses its 5' → 3' exonuclease activity. coli is enzymatically cleaved by the protease subtilisin. The Klenow fragment is a large protein fragment produced when DNA polymerase I from E. Functional domains in the Klenow Fragment (left) and DNA Polymerase I (PDB).
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9/12/2023 0 Comments Juicebox 32 proIn some locations, this could cover the full cost of the unit as well as the full cost of installation.įor more information, p lease give us a call at 88 or by our Live Chat to learn more about these potential savings. There may be rebates and incentive money available in your area along with federal tax credits. Allows for the ability to set pricing, check usage rates and will be visible to the public via the JuiceNet App. For example, you could set this unit to be free to your employees while charging customers a fee for use. JuiceNet Enterprise $150 per unit a year includes user authentication, additional payment options, advanced energy management and reportingįully control all aspects of the charging station. ![]() JuiceNet Business $95 per unit a year includes basic station control and reporting.JuiceNet software options (REQUIRED FOR EACH CHARGER).Control charging times and the individuals allowed to use the system.A built-in indicator light shows you when the EVSE is in charging mode. Of course, when you start charging, the time will vary depending on the EV model and battery level. Since the charging station offers 32 amps of power at full power, it can charge your car in just 4 hours. Ability to monetize the charge with a Pay-To-Charge mobile App The JuiceBox Pro 32 is rated at 7.7 kilowatt-hours.WiFi enabled with smartphone/web remote control: on/off, scheduled timer, consumption history, load. The JuiceBox 32 has a tethered 7m cable with a Type 2 connector. Can be installed on single phase power for a 7.4kW, 32A rating. JuiceBox 32 is a 22kW, 32A 3 phase Electric Vehicle charger. (Sold by Professional Grade Products, Fulfilled by Amazon). 22kW 3 Phase or 7.4kW Single Phase Type 2 EV Charger. ![]()
9/12/2023 0 Comments Bowmasters online![]() There are definitely a lot of cards in Tales of Middle-earth that might be great for specific Commander players, but we’ve kept our list a little broader to make sure we’re not just talking about Commander and consider a more format-agnostic appeal.īest Tales of Middle-Earth cards MTG LOTRĬhecking out MTG's Lord of the Rings set, Tales of Middle-earth We’ve also got a common card in the mix - which feels fitting, because if The Lord of the Rings is anything, it’s a tale of how something unassuming can change the world. We don't need them! Our generator is as simple as it is effective, and will allow you to obtain free money gems characters Diamond membership to live exciting experiences and become one of the best players of BOWMASTERS in much less time.The best cards in Tales of Middle-Earth, Magic: The Gathering’s Lord of the Rings set, don’t just include the characters we all know and love from the films, which is always good. 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TheKingPlayers□ Get Free Resources with this Generator! ![]() These are cell-damaging molecules which lead to disease and premature ageing. ‘Antioxidant’ is a buzzword in health and wellness, and anything with proven antioxidant properties is worth our attention.Īntioxidants work by scavenging oxidants, also known as free radicals. More research is needed on whether drinking liquid chlorophyll can help clear the skin from within – although remember that countless skin-loving foods such as spinach, spring greens, kale and cabbage are high in chlorophyll, so there’s method in the madness. 14īut what about the online trend of starting the day with a glass of liquid chlorophyll? Sensitive skin is a key cause of premature skin ageing, so it makes sense that anything which has soothing properties is good news for your skin. ![]() 13Ĭhlorophyll also holds soothing properties. Perhaps the most well-known potential chlorophyll benefit is a clearer complexion, but can you sip your way to glowing skin with chlorophyll?ĭue to its antibacterial properties, chlorophyll can help fight breakouts and other bacterial skin concerns when applied topically. More recent studies have also demonstrated chlorophyll’s antibacterial action against various microbes. This antibacterial action was confirmed during studies on different bacteria strains in the 1950’s. 10Ĭhlorophyll is able to slow and prevent the growth of various types of bacteria that may be harmful to health. 9 Another mechanism thought to support wound healing is by limiting harmful bacterial growth which can slow the speed at which a wound heals. It’s thought this is due to its ability to stimulate cell production, which is essential to heal skin following damage. ![]() It supports processes involved in healing woundsĬhlorophyllin (a semi-synthetic chlorophyll derivative which includes copper – commonly used in chlorophyll supplements) can promote and accelerate wound healing.6 This has the benefit of boosting giving us our energy. It’s been theorised that due to its chemical similarity to haemoglobin, chlorophyll can mimic haemoglobin in our bodies, helping rebuild our red blood cells and enabling them to carry more oxygen around the body. It can support our red blood cells, creating more energyĬhlorophyll has a similar chemical makeup to haemoglobin, the substance in red blood cells which carries oxygen around the body.Taking the green plant supplement reduced the participants’ cravings for sweets and chocolate, which could partially explain the weight loss effects seen in the study.Īnother finding was that taking the chlorophyll supplement increased feelings of satisfaction and fullness by releasing a gut hormone which tells the brain it has had enough. A study found that taking a 5g green plant membrane supplementation (which included chlorophyll) for 90 days led to weight loss. 9/12/2023 0 Comments Thanos actor potentialSpoiler alert: that version of the team parted ways at the end of the movie, with Star-Lord (Chris Pratt) returning to earth after his four decade hiatus, and the others dispersing across the stars. We reunited with the Guardians in Guardians of the Galaxy Vol. ![]() Rumours abound as to who they're gonna cast for those parts, but we're not ones to speculate (not until we ourselves have some more solid info, at least). Fantastic in Doctor Strange in the Multiverse of Madness. We know that Marvel's first family are going to be formally introduced to the MCU in 2024, well in time for that Avengers duology, after John Krasinski had a scene-shredding cameo as Mr. Well, there it is - it's not just about the Avengers anymore. But hey - at least we've got something of an idea… Will the Fantastic Four, Guardians of the Galaxy, and the Thunderbolts be in The Kang Dynasty and Secret Wars? Look, there're gonna be dozens of curveballs in the interim, so there's almost zero chance that this is gonna be the right lineup. Then there's Riri “Ironheart” Williams (Dominique Thorne), the teen genius in an Iron Man-esque metal exoskeleton who came in clutch in the battle between Wakanda and Namor's Talokan. Marvel (Kamala Khan, played by Iman Vellani) isn't Captain Marvel's understudy per se, but they are set to team up in The Marvels this year. Then there're the protegés of the present Avengers: it's well within the realm of possibility that America Chavez (Xochitl Gomez), the powerful multiverse jumper introduced in Doctor Strange 2, will be a central Avenger by the time The Kang Dynasty rolls around. And he's an Avenger in some of the big comic storylines. One hotly anticipated title is Blade, introducing Mahershala Ali's titular vampire killer the TV series Daredevil: Born Again, coming in 2024, is set to bring Charlie Cox's blind super-fighter into the mainstream Marvel fold, following appearances in She-Hulk and Spider-Man: No Way Home. As if you needed the reminder.At Comic-Con last July, Marvel added a swathe of new series and movies to their slate, along with dating some of their previously announced projects. And you can next see her, beginning on Thursday night, in Avengers: Infinity War. We will have our full interview with Gillan about The Party’s Just Beginning (and a little Jumanji 2) later this week. The photo of which the actor speaks is above. Because when I worked on Guardians, we really did become like a family, you know? Then we went into Avengers, and it was like we were meeting all of our cousins for the first time, and it was amazing.” “Yeah, and it sort of feels like a giant family portrait when we did that class photo. “It does feel a little bit like a graduation,” Gillan laughed. In both movies, she has shot extensive scenes as Nebula, the daughter of Avengers Big Bad Thanos (Josh Brolin) who is also, as per director James Gunn, “the Boba Fett of the MCU.” In fact, Gillan likened the whole release of these films to something like a graduation. Instead Gillan asserts the directors, Anthony and Joe Russo, personally scribbled down a fake version of the screenplay to confuse the actors and throw potential leakers off.īut full script, no script, or even a fake script, Gillan is jazzed to reach this milestone for Marvel Studios’ Marvel Cinematic Universe, which 10 years in is having its own kind of homecoming with the next two Avengers films. So we’re all just like, we can’t wait.” Further the Scottish filmmaker went on to add while only the “main” stars of Avengers: Infinity War received a full script, there is a chance none of them even read the real final version of the film penned by Christopher Markus and Stephen McFreely. I don’t think anyone has seen the movie anywhere in the world until-it’s happening on Monday for the first time. I’m in the same position as everybody else. “I haven’t seen the movie, nor have I read the entire script,” Gillan said with a hint of amazement about the whole process. Indeed, when we sat down with Karen Gillan at the Tribeca Film Festival this weekend to discuss her directorial debut, the hauntingly intimate The Party’s Just Beginning, the actor confided to us that she never read a full script for Avengers 3… even while she was shooting Avengers 4. And that includes the actors who star in it too. In fact, with the North American premiere for the film Monday night-as well as the only press screenings-people outside of Marvel Studios and Disney’s most exclusive inner-circle are actually going to be watching it for the first time. The week of Avengers: Infinity War is here. 9/12/2023 0 Comments Captain george pollard jr.![]() On January 28, 1821, the two remaining boats were separated. On January 23, Shorter died, while Sheppard and Samuel Reed, a man on Pollard’s boat, died on January 27 and 28, respectively. On January 20, Thomas had died, and his body was kept for food. ![]() Captain Pollard agreed to share the remaining provisions of his boat with them.īut it was no use, and on January 21, all the food supplies were exhausted. On January 14, 1821, Hendrick’s whaleboat that carried Lawson Thomas, Isaiah Sheppard, William Bond, Joseph West, and Charles Shorter ran out of food supplies. The survivors, desperate in their lack of food, resorted to cannibalism. On January 18, 1821, Peterson, one of the oldest crew members, died, followed almost immediately by Isaac Cole. On January 10, Chase’s whaleboat, carrying the crewmen Isaac Cole, Thomas Nickerson, Richard Peterson, and Benjamin Laurence, separated from the other whaleboats due to a squall. As per custom, Joy was sewn into his own clothes and later buried in the sea. On January 10, 1821, Joy died, and Obed Hendricks took charge of his boat. The health condition of Matthew Joy worsened with time, and he wanted to rest on the boat of Captain Pollard. The men were starting to die out of hunger and thirst. Captain Pollard gave an order to launch three whaling boats, which managed to successfully corral a number of whales. A Date with Destinyīack in open water, the crew of the Essex next spotted a sperm whale in November 1820. The entire crew of the Essex was upset about the fire incident, and a crewmember called Thomas Chapple later admitted to setting the fire. When the crewmen had reached the safety of the Essex, they could see that the whole island was on fire. Ernest Shackleton – Surviving Antartica.Taken by the Ice: What Became of the Franklin Expedition Crew?. ![]() Soon, it went out of their control, and they had to run through flames in order to save their lives and escape. As it was a dry season, and a fire started by the shore party started burning and spreading very quickly. Not content with removing the tortoises from Charles Island, the crew managed to torch it as well. The sailors believed that the tortoises could survive without food and water for nearly a year, they were collected alive and left to roam around in the ship. The tortoises stacked well in the ship’s hold and provided plenty of meat, weighing somewhere around 100 to 800 pounds. Giant tortoises were considered ideal provisions (Matthew Field / CC BY-SA 3.0 ) The crewmembers collected some 300 giant turtles for their food, and then on October 22, while heading towards Charles Island, they collected more than 60 tortoises as well. The ship stopped at Galápagos in October 1820 in order to stock up the provisions and repair some serious leaks that had appeared. In September 1820, one of the sailors, Henry DeWitt, left at Atacames in Ecuador, thereby reducing the number of crew members to 20 men. The crew of the Essex were able to catch a sperm whale every five days, collecting about 450 barrels of oil before rough conditions forced them west. Their fate started changing after reaching the region near Peru. For several months, they patrolled up and down the Chilean coastline but sighted only a few whales. However, as the region was fished out, they headed towards the Pacific with the hope of better luck, rounding Cape Horn in January 1820. The crew was even successful in catching their first sperm whale near Brazil. There the Essex was repaired and again set off towards South America. Lucky once again, the ship reached Cape Verde Island at the end of September. For about 20 years, all the voyages of The Essex had been successful, and often it was called a lucky vessel, popular with her crews.Ĭaptain Pollard opted to carry on the voyage without stopping to repair or replace the damaged whaleboats. The Essex was an American whaleship that was launched back in 1799. Obviously, this was not a normal occurrence – the idea of a whale attacking a ship was as sensational and shocking to Moby Dick’s 1851 readership as the story of a giant shark attacking beachgoers in Amity Island a century later. The Essex actually sank due to a whale attack, providing inspiration for Melville to write his book some 20 years later. One of the prominent stories among them was that of the Essex, a whaleship operating in the early 19th century. Well, while the murderous, gigantic white whale in the novel of Melville was a fictional one, Melville drew inspiration from a number of real-life horror stories. Have you read the novel named Moby Dick by Herman Melville? If you have read it, you must be surely wondering whether there was real Moby Dick or not. 9/12/2023 0 Comments Black light switch covers![]() ![]() The subplate doesnt show once the outer cover is snapped over it. Taking care of the customer is Lutron’s first principle and our number one priority. They can be used with any rocker device, but theyre made to cover Touch Plates line of Ultra low voltage light switches. Widest Selection Of Decorative Ceramic Light Switch Covers - Outlet Covers Light Switch Plates, Outlet Covers.period.Whether you are looking to reduce energy costs, increase comfort, or manage light control solutions, Lutron products offer the flexibility you need with the energy savings you want.Ĭonnected lighting, shades, and hand-crafted controls that elevate everyday life. : Black Light Switch Covers 1-48 of over 1,000 results for 'black light switch covers' Results ENERLITES Toggle Light Switch Wall Plate, Standard Size 1-Gang 4.50' x 2.Oversized 2 Rocker Switch Plates for 2 Decora Devices - Black. Oversized 2 Gang Outlet Switch Plates for 4 Sockets - Black. Oversized 2 Toggle Switch Plate Covers - Black. Contact Support 24/7 844-LUTRON1 (588-7661) Extra Large Blank Oversized Light Switch Cover 6.4 x 4.5 - Black. ![]() Lutron Headquarters & Lighting Control Institute.Serena Shades Mon-Fri: 8 AM – 8 PM (EST).Discussion Forum Board of Fawn Creek Montgomery County Kansas, US. Technical Documentation & Support Overview TOPIX, Facebook Group, Craigslist, City-Data Replacement (Alternative). 9/11/2023 0 Comments Toy submarine yellow big windows![]() I thought it might the stupid action of the week, but not at all. I drilled each hole to 7,5 mm diameter with an electric drill, and it worked perfectly, much to my surprise. Then I drew the correct window sizes in AutoCAD and printed four sets with centre marks, taped them in place on both sides and drilled a 1 mm hole in each centre. One of the two versions of decal window sets seems to be quite good in measurement, so I used those to get everything to line up. This is after moving the cut bits around. ![]() I begun taping the strip in place, but it was immediately clear that I had to shorten it towards the front and extend it before the split at the rear. It turned out to be identical to the right side, except of course that's mirrored, but good to know. I made a copy of the sheet and cut the strip for the left side out. All references are a bit conflicting so I decided to work out a compromise trying to use the red lines from the decal sheet. The next task was to determine exactly where the windows (port holes?) should be placed. Then I block sanded the whole area to make everything smooth and level and rescribed them. When filed to shape and fit they could finally be glued in place reasonably well. I added material in their openings and also extended the hatches themselves 2,5 mm at the bottom edge to give me something to work with. As I mentioned their fit, unless you are satisfied to open them under water in the bath tub, leaves about everything to be desired. The first thing I had to do was to fit the two side hatches. I'm not sure the film version is perfectly accurate to the boat used in the real battle, but at least it should be quite close in general. References for details are a bit difficult. Well, that's what's in the box of shame on the shelf. Eyes for toy animals, but of course I only need the glass part. I never got round to do anything to it though.Īnd here we have what I wanted for those domed windows. I think it is much more like the real thing than what the kit provided. I found a three bladed R/C boat propeller from Graupner that can be used after clean up and paint. The four periscopes have been assembled, but they lack the same thing as the side windows clear parts in a pressure resistant shape, i.e. The hatches were an awful fit in their openings and I never got round to fixing that. I had filled in the holes in the bottom that were for the wheels and the hinges for the side hatches. Not the awful propeller that I needed to find a replacement for. Here are all the parts I discarded from the kit. There is much more work remaining than my memory said. I pulled the box from the shelf a few days ago to have a new look. Eventually I got hold of the window material, but nothing happened. I knew what I wanted, but couldn't find it, and then other things got in between, resin girls I think, and the project was sadly put aside. The "kit" provides them in two different forms of decals that are not useful when building the real boat. Nevertheless I started work in good mood and solved a few issues, but it soon stalled as I could not find a good source for the 16 pressure resistant round windows. I would need considerably stronger acid than usual to be able to enjoy that. It was the only thing I could find to use as a base, but when it arrived it was very much a toy thing in kit form, with wheels and a rubber powered propeller, probably designed for use in the bath tub. I started this project in the 2012 using the reissued kit/toy from MPC that Round 2 had recently made available. The idea was to build a model of the real thing, with corrected details and weathering. It's the famous yellow submarine from the battle of Pepperland that was fought in 1968. OK, I will try to pull myself together and bring this one home. ![]() I am also a Microsoft Most Valued Professional (MVP) for M365 Apps & Services. SharePoint, I help companies of all sizes better leverage Modern Workplace and Digital Process Automation investments. Locate Microsoft Office in the list Right-click on the Microsoft. Enter the following text in the run box appwiz. Ran into some problems? Write down your problem or question in the comment section below.įor professional help, send me a message through my contact page, and I’ll get back to you asap.Īs the Chief Solutions Architect at Mr. Hold the Windows key and press the R key. When you do any of these, the changes will take effect immediately. Similar to the last steps when doing this with a SharePoint site: On the next screen, go to the site collection features setting: The Default location of Microsoft Office programs are in C:Program FilesMicorosoft OfficeOffice14. On the next page, click the more settings button on the left: If your not planning on buying 365, I would Uninstall it.Click the gear icon at the upper-right corner.You also need to change the settings in OneDrive manually: Note: For more information on OneDrive, check out: How to Use OneDrive: The Ultimate Beginner’s Guide. Click the OK button to save the changesĮven if SharePoint and OneDrive are connected, it doesn’t mean that changes in a SharePoint site also get reflected.Change the option to open in the client application.Find the setting for opening documents in the browser.On the settings page, go to the advanced settings: On the next screen, click the more library settings link: Click the gear icon in the upper-right corner.Navigate first to the target document library.Well, you can also do this per document library: Note: For saving documents to SharePoint, check here: How to Save a Document Into SharePoint (From Word, Excel, and Powerpoint). Click the activate button on its right sideīut what if you only wanted to open the files in Office desktop apps by default for specific document libraries only?.Find the option to open documents in client applications by default When I go to open any of my Office 365 programs, I get the following: Application was unable to start correctly 0xc0000142.On the next page, find the site collection features settings: Then click Set this program as default and. On the next screen, click the view all site settings link: On Windows: Go to Settings > Apps > Default apps > Set default by app. ![]() By Jess Weatherbed, a news writer focused on creative. Click the gear icon on the upper-right corner Microsoft’s office apps and services are getting new Copilot features, available to 600 customers willing to pay for early access.If you want to open all documents in their desktop apps by default in all document libraries on your SharePoint site, follow these steps: This is why it will be better if you simply make the documents open in their respective desktop app by default. But what if you open tens of documents per day? It makes more sense to change the default settings if most of the documents you open must be in its desktop app. How to make documents open in their desktop apps by default 9/11/2023 0 Comments Community service block grant![]() ![]() New this year, residents can pick up a block party kit at their local BCYF community center. "Thank you to Mayor Wu for securing these Block Party grants, which ensure our neighborhoods maintain their vibrancy and that resources are brought out of City Hall and into our streets."Īdditionally, this year the City is again distributing block party kits for residents to borrow games and supplies for neighborhood events. "A major piece of effective community engagement and collaboration is providing our neighbors with financial tools to bring our communities together," said Gabriela Coletta, District 1 Boston City Councilor. The Office of Civic Organizing will provide permitting support for interested residents. More information about applying for the block party permit and tips on how to host a block party can be found here. Residents must also apply for a block party permit before submitting their grant application, as this can take 15 business days to approve. Grant applications will be approved on a rolling basis through August 2023 to support block parties all summer. To be eligible, all block parties seeking funding must be free and open to the public. Residents must apply at least four weeks prior to their proposed event. “I want to encourage as many residents to apply for this funding opportunity to help them create joyful parties in their neighborhood.” “This grant program is such an exciting opportunity for community members to get financial assistance for the wonderful work they do each summer to create fun events to connect with their neighbors,” said Community Engagement Cabinet Chief Brianna Millor. I encourage anyone interested to apply and help make this summer a safe, enjoyable season in Boston.” “This year we’re trying to make it easier than ever to host a party by removing financial barriers. “Boston is a City of neighborhoods, and summer block parties give our residents a chance to get to know one another and enjoy the beauty of our communities,” said Mayor Michelle Wu. Today’s announcement builds on Mayor Michelle Wu’s commitment to fostering a fun, safe, and family-friendly summer in Boston. Additionally, the City will again be offering block party kits this summer for community members to borrow and use at their gatherings. Applicants should fill out this form with their contact information, date of the block party, and requested use of funds. Residents and community groups who apply for the mini-grants can receive up to $750 for their gathering. Today, Mayor Michelle Wu and the City of Boston’s Community Engagement Cabinet announced applications are now open for block party grants to purchase food, party supplies, lawn games, and other items for summer block parties to make it easier and more affordable for neighborhoods to hold community gatherings. |
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